Vector control is primordial and very essential means for controlling transmission of filariasis, malaria, Japanese encephalitis and dengue in human society. Over the last few decades, there is growing realization that alternate methods to synthetic chemical control needs to be studied and perfected. Several control strategies have been adopted to control diseases transmitted by mosquitoes. Mosquito control programs worldwide have been evaluating the feasibility to implement biological control strategies by using Bacillus sphaericus (Bs). A comprehensive review cum research data is presented here to assess the potentiality of Bs in mosquito control operation. The major advantages of Bs are reduced application cost, safety to environment, human beings, animals and other non-target organisms. This paper explores the importance of Bs bacterial toxin in controlling vector mosquitoes.