Proteins – structure, properties and their separation by SDS-polyacrylamide gel electrophoresis

Vikas Kumar Roy, N. Senthil Kumar, G. Gurusubramanian

Science Vision 12 (4), 170-181 | Published on 31 December 2012 |

Abstract

This paper deals with three basic aspects: 1) basics of protein structure in relation to physico-chemical characterization, protein stability, importance of disulfide bond and their structure; 2) basic principles involved in polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE and the rationale behind the use of disc electrophoresis; and 3) detailed protocol involved in SDS-PAGE in terms of reagent preparation, sample preparation and loading, staining and destaining, calculation of relative mobility and pairwise comparisons between species.

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