The present study is an attempt to document the traditional practice and knowledge of medicines/naturopathy among the Mizo ethnic group of Lunglei district, Mizoram. A total of 82 medicinal plant species belonging to 42 families and 76 genera, as well as 17 animal species was documented along with their parts used, methods of preparation and types of ailments treated. The present study revealed that there is a positive relationship between age and traditional knowledge and practice; while a negative relationship between educational level and traditional knowledge and practice was observed. Ethno-medicinal knowledge and practices of the Mizo and the medicinal plant species are under serious threat due to various factors. Therefore, urgent attention towards their conservation and sustainable utilization is needed.