Molecular phylogenetic relationships among members of the odonate genus Orthetrum (Odonata: Anisoptera: Libellulidae) were examined using 403 bp of mitochondrial COI. The support for monophyly of the Orthetrum was found in some studies with unresolved complexity. The O. sabina, O. serapia and O. trincaria formed a seperate and distinct group from the morphological analysis. We analysed the COI sequences of 22 species of Orthetrum using MEGA6. The p-distance between the members and the rate of transitional and transversional substitution was generated. The analysis indicated that the Orthetrum are monophyletic and O. sabina and O. trinacria formed a disticnt and a seperate group.