The spectroscopic properties of Ho3+ in SiO2-TiO2 were investigated using optical absorption and fluorescence studies. The intensities of the electric dipole transitions of Ho3+ in the SiO2-TiO2 doped glasses were calculated from the absorption spectra in the visible region. From the experimental values of the oscillator strengths and calculated matrix elements, the Judd-Ofelt intensity parameters were calculated by least square analysis. The fluorescence spectral distribution of Ho3+ has been recorded using 370nm excitation source. The emission bands observed near blue and green regions were assigned as 5G5, 5G6→5I8, 5F3→5I8 and 5F4, 5S2 →5I8 radiative transitions.